DNA Extraction and Purification
Amsbio LLC MagSi-DNA mf
MagSi-DNA mf is ideal for developing your own application, such as: - Isolation of genomic, mitochondrial, or viral DNA from whole blood, cell lysates, human, animal, or plant tissue; isolation of RNA - Isolation of genomic, plasmid, or phage DNA from bacterial cultures and bacteria from clinical samples (blood, stool, swabs, etc.) - Clean-up of DNA from enzymatic reactions or chromatin immunoprecipitation (ChIP) | MagSi-DNA mf consists of magnetic silica beads with a highly dense core of magnetite. Due to their ferrimagnetic properties, the beads typically collect within 10 seconds in a magnetic field, but quickly loose magnetic remanence and are easily resuspended. The beads have minimal adhesion effects to plastics, making them perfectly suitable for use in microfluidic systems. The small particle size offers a very large active surface area for binding of nucleic acids, and the storage buffer has been optimized for maximum suspension time and ease of resuspension.
Biochain Institute Inc Genomic DNA - Progressive Supranuclear Palsy: Brain: Occipital Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Tumor Tissue: Kidney Tumor, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Progressive Supranuclear Palsy: Brain: Frontal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Research Products International Corp Genomic DNA Clean & Concentrator-10 Kit, 25 Preps
The Genomic DNA Clean & Concentrator-10 (gDCC) is a gDNA clean up kit that provides quick, 5 minute recovery of ultra-pure, large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage, WGA DNA, etc.) from any enzymatic reaction or impure preparation (e.g., Proteinase K digestion). There is no need for organic denaturants, chloroform, or messy precipitations: simply add the specially formulated ChIP DNA Binding Buffer to a sample and then transfer the mixture to the supplied Zymo-Spin Column. Eluted DNA is suitable for sequencing, PCR, endonuclease digestion, and other enzymatic procedures. This gDNA clean up kit is also compatible with smaller DNAs (50 bp to 10 kb) from PCR, digestions, crude plasmid preparations, cDNA synthesis, etc.
Quick, 5-minute clean-up of large-sized DNA from any enzymatic reaction or impure preparation without messy precipitations.
Zymo Research Corporation Quick-DNA HMW MagBead Kit (96 prep)
Innovative reagents and Zymo Researchs unique system allow for a simple Bind, Wash, & Elute procedure that is unmatched in providing ultra-pure and high yield DNA of > 150 kb size. Isolated DNA is ready for immediate use in sensitive downstream applications including long read (Oxford Nanopore™) and NGS sequencing, qPCR, arrays, and methylation analysis.
New England Biolabs, Inc. Monarch® gDNA Wash Buffer - 60 ml
Monarch gDNA Wash Buffer is a component of the Monarch Genomic DNA Purification Kit. It is an ethanol-based, low salt buffer that allows for complete removal of chaotropic salt and cell components in just 2 wash steps, enabling the purification of highly-pure gDNA.
- A component of the Monarch Genomic DNA Purification Kit
- An ethanol-based, low salt buffer that allows for removal of chaotropic salt and cell components
- Supplied as a concentrate
- Monarch kit components are also available separately for added convenience
LGC GENOMICS LLC (Lucigen) sbeadex™ maxi Plant DNA Purification Kit (80-100 mg starting material), 960 purifications
Highly efficient, magnetic bead based purification of DNA from plant samples.
Research Products International Corp ZymoPURE II Plasmid Gigaprep, 5 Preps
Part of the ZymoPURE Plasmid Kits collection, the ZymoPURE II Plasmid Gigaprep kit provides the fastest and simplest method available to efficiently isolate up to 10 mg of transfection grade plasmid DNA from E. coli. Utilizing a modified alkaline lysis in conjunction with a novel binding system, the ZymoPURE II Plasmid Gigaprep kit can process up to 2.5 L of culture in less than 50 minutes. This remarkable kit results in significantly more plasmid DNA while providing drastically reduced processing times. The plasmid DNA is bound onto a column with a vacuum instead of a lengthy gravity flow column. Additionally, there is no ethanol precipitation step required and the elution is performed using a microcentrifuge. The recovered plasmid DNA is highly concentrated (up to 3 µg/µl) , EndoZero, vaccine-grade, and transfection-ready.
Macherey-Nagel NucleoBond Finalizer Large Plus, 20 finalizers
NucleoBond Finalizer Large Plus (20 sets) filters for plasmid DNA precipitation, to be used with NucleoBond Xtra Maxi kits - 20 NucleoBond Finalizers Large, 20 syringes of 30 mL, and 20 syringes of 1 mL
Omega Biotek Inc Endo-Free Plasmid DNA Maxi Kit, 25 preps
Endo-Free Plasmid DNA Maxi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. Following lysate clearance, endotoxins are removed, and the protocol follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA.
Biochain Institute Inc Genomic DNA - Plant Normal Tissue: Orange, 10 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Zymo Research Corporation Zyppy™ Plasmid Miniprep Kit (50 Preps)
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.